| 質粒名稱: | pMXs-Klf4 |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 13370 |
| 存儲實驗室: | Shinya Yamanaka |
|
載體骨架基本信息 |
|
| 載體名稱: | pMXs |
| 載體出品公司: | -- |
| 載體抗性: | 氨芐青霉素 |
| 空載體大小: | 4600 bp |
| 完整質粒大小: | -- |
| 載體修飾: | -- |
| 載體類型: | 哺乳動物表達，逆轉錄病毒 |
| 篩選標記: | -- |
| 高拷貝/低拷貝: | 高拷貝 |
| 生長條件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名稱: | Kruppel-like factor 4 (gut),Klf4 |
| 物種來源: | M. musculus (mouse) |
| 插入大小: | 1425 bp |
| 基因突變: | None |
| GenBank編號 | -- |
| 啟動子 | -- |
| 標簽/融合蛋白: | -- |
| 克隆方法: | Unknown |
| 5' 克隆位點: | -- |
| 3' 克隆位點: | -- |
| 5' 測序引物 | pMX-S1811 (5'-GACGGCATCGCAGCTTGGATACAC-3') |
| 3' 測序引物 | LNCX-3 (5'-ACCTACAGGTGGGGTCTTTCATTCCC-3') |
|
文獻引用方法 |
|
| 材料和方法部分: | pMXs-Klf4 was a gift from Shinya Yamanaka (Addgene plasmid # 13370 ; //n2t.net/addgene:13370 ; RRID:Addgene_13370) |
| 參考文獻部分: | Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174 |
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pMXs-Klf4載體 pMXsKlf4質粒基本信息
pMXs-Klf4質粒圖譜 pMXsKlf4載體圖譜和pMXs-Klf4載體序列 pMXsKlf4質粒序列多克隆位點信息
pMXs-Klf4質粒 pMXsKlf4載體簡介
pMXs-Klf4質粒序列 pMXsKlf4載體序列
LOCUS pMXs-Klf4 6210 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Retroviral expression of mouse Klf4.
ACCESSION .
VERSION .
KEYWORDS pMXs-Klf4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6210)
AUTHORS Takahashi K, Yamanaka S
TITLE Induction of pluripotent stem cells from mouse embryonic and adult
fibroblast cultures by defined factors.
JOURNAL Cell. 2006 Aug 25. 126(4):663-76.
PUBMED 16904174
REFERENCE 2 (bases 1 to 6210)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..6210
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 120..589
/note="long terminal repeat from Moloney murine leukemia
virus"
protein_bind 906..930
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(2410..2434)
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 2485..2859
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind complement(2531..2547)
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
CDS complement(2869..3285)
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind complement(2885..2901)
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(2917..2939)
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
misc_feature 3344..3701
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
promoter 4312..4416
/gene="bla"
/label=AmpR promoter
CDS 4417..5277
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4635..4654)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5441..6029
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5930..5949
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6183..6200
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
241 cagtcaatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
721 ttctggagac taaataaaat cttttatttt atcgtcgacc actgtgctgg cggccgctcg
781 agtttaaata gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg
841 gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcagctgga
901 atatcaccac tttgtacaag aaagctgggt cggcgcgccc acccttacat ccactacgtg
961 ggatttaaaa gtgcctcttc atgtgtaagg caaggtggtc cgacctggaa aaggccctgt
1021 cgcacttctg gcactgaaag ggccggtgcc ctgtgtgttt gcggtagtgc ctggtcagtt
1081 catcggagcg ggcgaatttc cacccacagc cgtcccagtc acagtggtaa ggtttctcgc
1141 ctgtgtgagt tcgcaggtgt gccttgagat gagaactctt ggtataggtt ttgccacagc
1201 ctgcatagtc acaagtgtgg gtggctgttc ttttccgggg ccacgacctt cttcccctct
1261 ttggcttggg ctcctctggc aggcaggaac ccggtggcat gagctcttga taatggagag
1321 aggggacttg tgactgcatc tggtctggca ggaaaggagg gtagttgggc cccggatggg
1381 gatggaatcc tgggggaaga ggcaggccag ggtgacagtc cctgctgttc agcagttcct
1441 cgggactcag tgtaggggta gtcctggtgg ggagctgccg ccccaggggg aagtcgtgtg
1501 tgttgggccg gtggccgttg ctgagctggg gtccagcgct caaatgggcc tctagggacc
1561 ggctgaccgt gcaggacggg accgcctctt gcttaatctt ggggcacatg cgcggcgggc
1621 caccgctgta gggcgccact accacggggt ggctgccgtc tgggcttcct ttgctaacac
1681 tgatgaccga agggctgctg tactcgctgc caggggtggt cagagacgcc ttcagcacaa
1741 acttgcccat cagcccgcca cctggcggct gaggctgctg tggcggaatg tatactgggt
1801 ccaactccgg ccgcaggagc tcagccacga agccgcccga ggggctcacg tcattgatgt
1861 ccgccaggtt gaagggggcc gtgggaggtg gcgcagattc tcggctgtag aggagccctc
1921 cacctgtgtt gctggcagcc acgcccgggt cacccccggc ccggatcgga tagctgaagc
1981 tgcaggtgga gggcgcgctg gcagggccgc tgctcgctgg ggaagacgag gatgaagctg
2041 acgccgaggt ggtcacggtg gcggccaccg attcctggtg ggttagcgag ttggaaagga
2101 taaagtctag gtccaggagg tcgttgaact cctcggtctc cctccgggct aggagggccg
2161 ggttgttact gctgcaagct gcaccagctc cgccactctc caggtctgtg gccaccgtcg
2221 ccgccaggtc gtaggggcgg ccgggaagtg ggggaagtcg cttcatgtga gagagttcct
2281 cacgccaacg gttagtcggg gcacctgctg gacgcagtgt cttctccctt cccgccgggc
2341 cggacgcgaa cgtggagaag gacgggagca gagcgtcgct gacagccatg gtgaaggggg
2401 cggccgcgga gcctgctttt ttgtacaaac ttgtgatttc ccgtaccacc acactgggat
2461 ccttaattaa ctagctagat ccggcagtct agaggatggt ccacccccgg ggtcggcagc
2521 cttcacgtgg gcggcgtgta tccaagctgc gatgccgtct actttgaggg cggtgggggt
2581 ggtcagcagg actgtgtaag gtcctttcca gcgaggttct aggttcttag tctggtgtcg
2641 gcggacccac actgtgtcgc cgactcggta agggtgaggt accaccggtc ggtccagttg
2701 ttcttggtag gctgccgcca gaggtctcca gacttcgtgc tggactaagt agagagcctg
2761 taagtgagct tggagagagg ggctgttagt aactcttgtc atgtcagggt cagggaagtt
2821 tacaaggggc gggggtgccc catataagat ctcatatggc catatggggg cgcctagaga
2881 aggagtgagg gctggataaa gggaggatcg aggcggggtc gaacgaggag gttcaagggg
2941 gagagacggg gcggatggag gaagaggagg cggaggctta gggtgtacaa agggcttgac
3001 ccagggaggg gggtcaaaag ccaaggcttc ccaggtcacg atgtagggga cctggtctgg
3061 gtgtccatgc gggccaggtg aaaagacctt gatcttaacc tgggtgatga ggtctcggtt
3121 aaaggtgccg tctcgcggcc atccgacgtt aaaggttggc cattctgcag agcagaaggt
3181 aacccaacgt ctcttcttga catctaccga ctggttgtga gcgatccgct cgacatcttt
3241 ccagtgacct aaggtcaaac ttaagggagt ggtaacagtc tggccctaat tttcagacaa
3301 atacagaaac acagtcagac agagacaaca cagaacgatg ctgcagcaga caagacgcgc
3361 ggcgcggctt cggtcccaaa ccgaaagcaa aaattcagac ggaggcggga actgttttag
3421 gttctcgtct cctaccagaa ccacatatcc ctcctattag gggggtgcac caaagagtcc
3481 aaaacgatcg ggatttttgg actcaggtcg ggccacaaaa acggcccccg aagtccctgg
3541 gacgtctccc agggttgcgg ccgggtgttc cgaactcgtc agttccacca cgggtccgcc
3601 agatacagag ctagttagct aactagtacc gacgcaggcg cataaaatca gtcatagaca
3661 ctagacaatc ggacagacac agataagttg ctggccagct tacctcccgg tggtgggtcg
3721 gtggtccctg ggcaggggtc tcccgatccc ggacgagccc ccaaatgaaa gacccccgct
3781 gacgggtagt caatcactca gaggagaccc tcccaaggaa cagcgagacc acaagtcgga
3841 tgcaactgca agagggttta ttggatacac gggtacccgg gcgactcagt caatcggagg
3901 actggcgccc cgagtgaggg gttgtgggct cttttattga gctcggggag cagaagcgcg
3961 cgaacagaag cgagaagcga actgattggt tagttcaaat aaggcacagg gtcatttcag
4021 gtccttgggg caccctggaa acatctgatg gttctctaga aactgctgag ggctggaccg
4081 catctgggga ccatctgttc ttggccctga gccggggcag gaactgctta ccacagatat
4141 cctgtttggc ccatattcag ctgttccatc tgttcctgac cttgatctga acttttctat
4201 tctcagttat gtatttttcc atgccttgca aaatggcgtt acttaagcta gcttgccaaa
4261 cctacaggtg gggtctttca ttatgcaggt ggcacttttc ggggaaatgt gcgcggaacc
4321 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
4381 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
4441 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
4501 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
4561 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
4621 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
4681 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
4741 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
4801 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
4861 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
4921 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
4981 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
5041 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
5101 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
5161 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
5221 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
5281 tcagaccaag tttactcata tatactttag attgatttgc ggccggccgc aaaaggatct
5341 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
5401 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
5461 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
5521 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
5581 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
5641 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
5701 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
5761 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
5821 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
5881 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
5941 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
6001 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
6061 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
6121 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
6181 gcagcgagtc agtgagcgag gaagcggaag
//
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