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抗真菌劑測定瓊脂；殺真菌劑測定培養基 |
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Antifungal Assay Agar |
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非選擇性培養基 |
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for microbiology |
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ELITE-MEDIA（艾禮培養基） |
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M297-01、M297-02 |
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250g、500g |
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麥秸色粉末。 |
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淺黃色略不透明凝膠。 |
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密封，2-25°C保存。 |
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避免攝入、吸入、皮膚接觸。 |
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產品描述：
抗真菌測定瓊脂（Antifungal Assay Agar）用于使用管碟法（牛津杯法）或紙片擴散法測定藥品和其它 材料的抗真菌活性。
工作原理
真菌感染在過去的十年中急劇增加，經常伴隨系統性感染，如AIDS、癌癥患者和免疫能力降低的病人。不 幸的是，抗真菌藥物種類很少，真菌感染趨于快速產生對這些藥物的耐藥性。真菌感染的致死率遠高于細菌感染。因此，抗真菌劑的增加和抗 真菌劑的需求的增加，急需更快、更準確的抗真菌藥物篩選方法和藥敏試驗（susceptibility testing）方法。抗真菌劑測定瓊脂由Berger 和Lazecka研發，既作為基礎瓊脂也作為種子瓊脂，用于便捷地測定藥品和其它材料的抗真菌活性。該培養基是合成培養基，成分是已知的， 因此抑制性只能來自抗真菌劑，而不是養分耗竭。
用途：
抗真菌測定瓊脂被推薦用于測定藥品和其它材料的抗真菌活性。
配方與配制方法
|
成分 |
(g/L) |
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葡萄糖 |
50.000 |
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檸檬酸鈉 |
4.500 |
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磷酸氫二鈉 |
0.550 |
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檸檬酸 |
1.000 |
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酪蛋白胨 |
4.000 |
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鹽酸吡哆辛 |
0.00025 |
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維生素B1 |
0.00025 |
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肌醇 |
0.025 |
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泛酸鈣 |
0.0025 |
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煙酸 |
0.0025 |
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氯化鉀 |
0.425 |
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氯化鈣 |
0.125 |
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硫酸鎂 |
0.125 |
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氯化鐵 |
0.0025 |
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硫酸鎂 |
0.0025 |
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生物素 |
0.000008 |
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瓊脂 |
15.000 |
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pH 5.5±0.2 |
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配制方法：
1. 稱取75.76g本產品，用1000ml純水重懸。
2. 加熱使培養基完全溶解。
3. 121°C 滅菌15min。
4. 冷卻至45-50°C 時倒平板。
實驗方法
Cylinder plate method: This method was first devised by Abraham et al (4) and later modified by Schmidt and Moyer (5) and it depends upon diffusion of the antibiotic from vertical steel cylinders placed on the surface of inoculated agar medium. This produces zones of inhibition around the cylinder containing antibiotic solution depending upon the concentration of the antibiotic in the cylinder. This method is commonly employed in the assay of pharmaceutical preparations of Penicillin and other antibiotics. For assay, use Petri plates with 20 x100 mm dimension and stainless steel or porcelain cylinders with the outside diameter 8 mm, inside diameter 6 mm and length 10 mm. All dimensions should have a tolerance of 0.1 mm. The cylinders should be carefully cleaned to remove all the impurities. For assays requiring base and seed layer, the base layer is allowed to solidify first and then overlaid with the seed agar containing the proper concentration of the test organism. Most assays require base layer of 21 ml and seed layer of 4 ml. Generally 6 cylinders are used per plate. The cylinders are placed on inoculated plates at equal distance.
Paper-disc method: Paper discs with a diameter of 9 mm are impregnated with the antibiotic solution and placed on the culture medium. Antibiotic can also be applied to the disc after it has been placed on the medium. Plates containing a single layer of medium with 2 mm thickness may be used for these tests. All other steps are similar to the cylinder plate method.
